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- A mutation uncouples the tubulin conformational and GTPase cycles . . .
How this conformational cycle contributes to microtubule growing, shrinking, and switching remains unknown Here, we report that a buried mutation in αβ-tubulin yields microtubules with dramatically reduced shrinking rate and catastrophe frequency
- Geyer EA, et al. (2015) | SGD - Saccharomyces Genome Database
Here, we report that a buried mutation in αβ-tubulin yields microtubules with dramatically reduced shrinking rate and catastrophe frequency The mutation causes these effects by suppressing a conformational change that normally occurs in response to GTP hydrolysis in the lattice, without detectably changing the conformation of unpolymerized
- A mutation uncouples the tubulin conformational and GTPase cycles . . .
Geyer et al now show how this shape shifting can influence microtubule shrinking, by first identifying a mutation in yeast microtubule proteins that cause the proteins to remain straight even when GDP is attached
- A mutation uncouples the tubulin conformational and GTPase cycles . . .
Geyer et al now show how this shape shifting can influence microtubule shrinking, by first identifying a mutation in yeast microtubule proteins that cause the proteins to remain straight even when GDP is attached Next, powerful microscopes were used to make time-lapse videos of the mutated microtubules
- Publications | mysite
A mutation uncouples the tubulin conformational and GTPase cycles, revealing allosteric control of microtubule dynamics Geyer EA, Burns A, Lalonde BA, Ye X, Piedra FA, Huffaker TC, and Rice LM eLife 2015 Oct; 4:e10113 [Pubmed]
- Author response: A mutation uncouples the tubulin conformational and . . .
Semantic Scholar extracted view of "Author response: A mutation uncouples the tubulin conformational and GTPase cycles, revealing allosteric control of microtubule dynamics" by Elisabeth A Geyer et al
- Insights into allosteric control of microtubule dynamics from a buried . . .
Here, we sought to determine whether the microtubule-stabilizing effects (slower shrinking) of the β:T238A mutation we previously observed using yeast αβ-tubulin would generalize to mammalian microtubules
- Figures and figure supplements - cdn. elifesciences. org
A mutation uncouples the tubulin conformational and GTPase cycles, revealing allosteric control of microtubule dynamics Elisabeth A Geyer et al Geyer et al eLife 2015;4:e10113 DOI: 10 7554 eLife 10113 1 of 8 RESEARCH ARTICLE
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